Composite

Part:BBa_K3838345:Design

Designed by: Siyang Yu   Group: iGEM21_SZU-China   (2021-09-30)


YH-SOD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 534
    Illegal NheI site found at 147
    Illegal NheI site found at 170
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 534
    Illegal BglII site found at 577
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 534
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SOD's enzyme activity will beaffected by the label.


Source

The SOD gene is from Lactobacillus casei strain Lc18.

References